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ISO 14501 | IDF 171: 2021 Milk and milk powder — Determination of aflatoxin M1 content — Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography

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This document specifies a method for the determination of aflatoxin M1 content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder, i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 μg/kg for milk powder and 0,005 μg/kg for liquid milk. The limit of quantification (LOQ) is 0,1 μg/kg for milk powder and 0,01 μg/kg for liquid milk. The method is also applicable to low-fat milk, skimmed milk, low-fat milk powder and skimmed milk powder.

This third edition cancels and replaces the second edition (ISO 14501 | IDF 171:2007), which has been technically revised. The main changes compared with the previous edition are as follows: — the lack of detailed explanation in some clauses was leading to variability in the way the method was executed from one laboratory to another. Practical information from skilled end users familiar with routine analysis using this protocol was taken into account in this revision to clarify those ambiguous clauses.

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